A function-blocking CD47 antibody modulates extracellular vesicle-mediated intercellular signaling between breast carcinoma cells and endothelial cells.

ABSTRACT: Tumor cells release extracellular vesicles (EVs) into the tumor microenvironment that may facilitate malignant progression and metastasis. Breast carcinoma EVs express high levels of the thrombospondin-1 and signal regulatory protein-α receptor CD47, which is the target of several experimental therapeutics currently in clinical trials. We analyzed changes in gene expression and function in human umbilical vein endothelial cells (HUVEC) induced by treatment with EVs derived from breast carcinoma cells and the effects of the function-blocking CD47 antibody B6H12 on the resulting intercellular communication. CD47⁺ EVs exhibited greater uptake by HUVEC compared to CD47^- EVs, but the CD47 antibody did not inhibit their uptake. Global and targeted analyses of transcripts de

SUBMITTER: Kaur S 

PROVIDER: S-EPMC5842190 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature