Coordinated transcription of ANRIL and P16 genes is silenced by P16 DNA methylation.

ABSTRACT:

Objective

To investigate the relationship between the transcription of ANRIL, P15, P14 and P16 at the same locus and the regulation mechanism of ANRIL.

Methods

Publicly available database of Cancer Cell Line Encyclopedia (CCLE) was used in bioinformatic analyses. Methylation of CpG islands was detected by denaturing high performance liquid chromatography (DHPLC). Gene transcript levels were determined using quantitative real-time polymerase chain reaction (qRT-PCR) assays. An engineered P16-specific transcription factor and DNA methyltransferase were used to induce P16-specific DNA demethylation and methylation.

Results

The expression level of ANRIL was positively and significantly correlated with that of P16<

SUBMITTER: Gan Y 

PROVIDER: S-EPMC5842237 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature