Colorectal cancer liver metastases organoids retain characteristics of original tumor and acquire chemotherapy resistance.

ABSTRACT:

Background

Colorectal cancer (CRC) liver metastasis is highly unfavorable for patient outcome and is a leading cause of cancer-related death. Pre-clinical research of CRC liver metastasis predominately utilizes CRC cell lines grown in tissue culture. Here, we demonstrate that CRC liver metastases organoids derived from human specimens recapitulate some aspects of human disease.

Methods

Human CRC liver metastases pathological specimens were obtained following patient consent. Tumor disaggregates were plated and organoids were allowed to expand. CRC markers were identified by immunofluorescence. Stem cell genes were analysed by QPCR and flow cytometry. Response to drug therapy was quantified using time-lapse imaging and MATLAB analysis.

Results

Organoids showed global e

SUBMITTER: Buzzelli JN 

PROVIDER: S-EPMC5842239 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature