Novel hemostatic biomolecules based on elastin-like polypeptides and the self-assembling peptide RADA-16.

ABSTRACT:

Background

Safe and effective hemostatic materials are important for reducing mortality resulting from excessive hemorrhage. In this work, new biomaterials with hemostatic effects were created by fusing the gene coding for RADA-16, a self-assembling peptide with the sequence RADARADARADARADA, to the 3'-end of the open reading frame (ORF) encoding elastin-like polypeptides through gene recombination.

Results

The fusion proteins, termed 36R, 60R and 96R, were solubly over-expressed in Escherichia coli BL21 (DE3) based on genetic manipulation of the high-efficiency prokaryotic expression vector pET28a (+) and bacterial transformation. Western Blot analysis showed that the over-expressed proteins were the target fusion proteins. The target proteins 36R with 94.72% purity, 60R wi

SUBMITTER: Yang S 

PROVIDER: S-EPMC5842521 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature