A synbio approach for selection of highly expressed gene variants in Gram-positive bacteria.

ABSTRACT: BACKGROUND:The market for recombinant proteins is on the rise, and Gram-positive strains are widely exploited for this purpose. Bacillus subtilis is a profitable host for protein production thanks to its ability to secrete large amounts of proteins, and Lactococcus lactis is an attractive production organism with a long history in food fermentation. RESULTS:We have developed a synbio approach for increasing gene expression in two Gram-positive bacteria. First of all, the gene of interest was coupled to an antibiotic resistance gene to create a growth-based selection system. We then randomised the translation initiation region (TIR) preceding the gene of interest and selected clones that produced high protein titres, as judged by their ability to survive on high concentrations of antibiotic

SUBMITTER: Ferro R 

PROVIDER: S-EPMC5842541 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature