Proteomic study revealed antipsychotics-induced nuclear protein regulations in B35 cells are similar to the regulations in C6 cells and rat cortex.

ABSTRACT:

Background

Based on accumulating evidence, the regulation of protein expression by antipsychotic drugs (APDs) might be closely related to the control of psychotic symptoms when these drugs are used to treat mental disorders. The low quantity of nuclear proteins in the cell hinders their detection because signal for rare proteins are masked in most proteomic detection systems.

Methods

Nuclear proteins fractionated from APD-treated B35 cells were labeled with iTRAQ and detected by LC/MS/MS to investigate APD-induced alterations in nuclear protein expression. Western blot, immunofluorescent cell staining, and immunohistochemical staining were applied to validate the findings.

Results

The expression of ADP/ATP translocase 2, heat shock cognate 71 kDa protein, histone H1.2

SUBMITTER: Li T 

PROVIDER: S-EPMC5842604 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature