Pharmacological activation of TAZ enhances osteogenic differentiation and bone formation of adipose-derived stem cells.

ABSTRACT:

Background

Adipose-derived stem cells (ADSCs) are an attractive cell source for bone tissue engineering and have great potential for bone regeneration and defect repair. The transcriptional coactivator with PDZ-binding motif (TAZ) has been demonstrated to modulate osteogenic and adipogenic differentiation of mesenchymal stem cells. However, its roles during ADSC differentiation and therapeutic potentials for bone regeneration have as yet not been well established.

Methods

TAZ expression was measured during osteogenic differentiation of ADSCs in vitro. Both loss-of-function and gain-of-function approaches by TAZ knockdown or enforced overexpression were utilized to determine its functions during osteogenic differentiation of ADSCs. TM-25659, a chemical activator of TAZ, was u

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC5842656 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature