Hypoxia imaging with ¹⁸F-FAZA PET/CT predicts radiotherapy response in esophageal adenocarcinoma xenografts.

ABSTRACT:

Background

Esophageal cancer is an aggressive disease with poor survival rates. A more patient-tailored approach based on predictive biomarkers could improve outcome. We aimed to predict radiotherapy (RT) response by imaging tumor hypoxia with ¹⁸F-FAZA PET/CT in an esophageal adenocarcinoma (EAC) mouse model. Additionally, we investigated the radiosensitizing effect of the hypoxia modifier nimorazole in vitro and in vivo.

Methods

In vitro MTS cell proliferation assays (OACM5 1.C SC1, human EAC cell line) were performed under normoxic and hypoxic (< 1%) conditions: control (100 μL PBS), nimorazole, irradiation (5, 10 or 20 Gy) with or without nimorazole. In vivo, subcutaneous xenografts were induced in nude mice (OACM5 1.C SC1). Treatment was given daily for 5 cons

SUBMITTER: Melsens E 

PROVIDER: S-EPMC5842657 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature