Optimisation of growth conditions for ovine airway epithelial cell differentiation at an air-liquid interface.

ABSTRACT: Respiratory tract infections are of significant concern in the agriculture industry. There is a requirement for the development of well-characterised in vitro epithelial cell culture models in order to dissect the diverse molecular interactions occurring at the host-pathogen interface in airway epithelia. We have analysed key factors that influence growth and differentiation of ovine tracheal epithelial cells in an air-liquid interface (ALI) culture system. Cellular differentiation was assessed at 21 days post-ALI, a time-point which we have previously shown to be sufficient for differentiation in standard growth conditions. We identified a dose-dependent response to epidermal growth factor (EGF) in terms of both epithelial thickening and ciliation levels. Maximal ciliation levels were obs

SUBMITTER: O'Boyle N 

PROVIDER: S-EPMC5843276 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature