Whole-Genome Sequence Accuracy Is Improved by Replication in a Population of Mutagenized Sorghum.

ABSTRACT: The accurate detection of induced mutations is critical for both forward and reverse genetics studies. Experimental chemical mutagenesis induces relatively few single base changes per individual. In a complex eukaryotic genome, false positive detection of mutations can occur at or above this mutagenesis rate. We demonstrate here, using a population of ethyl methanesulfonate (EMS)-treated Sorghum bicolor BTx623 individuals, that using replication to detect false positive-induced variants in next-generation sequencing (NGS) data permits higher throughput variant detection with greater accuracy. We used a lower sequence coverage depth (average of 7×) from 586 independently mutagenized individuals and detected 5,399,493 homozygous single nucleotide polymorphisms (SNPs). Of these, 76% or

SUBMITTER: Addo-Quaye C 

PROVIDER: S-EPMC5844295 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature