Base-Resolution Analysis of DNA Methylation Patterns Downstream of <i>Dnmt3a</i> in Mouse Naive B Cells.

ABSTRACT: The DNA methyltransferase, Dnmt3a, is dynamically regulated throughout mammalian B cell development and upon activation by antigenic stimulation. Dnmt3a inactivation in hematopoietic stem cells has been shown to drive B cell-related malignancies, including chronic lymphocytic leukemia, and associates with specific DNA methylation patterns in transformed cells. However, while it is clear that inactivation of Dnmt3a in hematopoietic stem cells has profound functional effects, the consequences of Dnmt3a inactivation in cells of the B lineage are unclear. To assess whether loss of Dnmt3a at the earliest stages of B cell development lead to DNA methylation defects that might impair function, we selectively inactivated Dnmt3a early in mouse B cell develo

SUBMITTER: Duncan CG 

PROVIDER: S-EPMC5844302 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature