The Hidden Genomic and Transcriptomic Plasticity of Giant Marker Chromosomes in Cancer.

ABSTRACT: Genome amplification in the form of rings or giant rod-shaped marker chromosomes (RGMs) is a common genetic alteration in soft tissue tumors. The mitotic stability of these structures is often rescued by perfectly functioning analphoid neocentromeres, which therefore significantly contribute to cancer progression. Here, we disentangled the genomic architecture of many neocentromeres stabilizing marker chromosomes in well-differentiated liposarcoma and lung sarcomatoid carcinoma samples. In cells carrying heavily rearranged RGMs, these structures were assembled as patchworks of multiple short amplified sequences, disclosing an extremely high level of complexity and definitely ruling out the existence of regions prone to neocentromere seeding. Moreover, by studying two well-differentiated li

SUBMITTER: Macchia G 

PROVIDER: S-EPMC5844343 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature