A high-throughput 3' UTR reporter screening identifies microRNA interactomes of cancer genes.

ABSTRACT:

Introduction

Despite the established contribution of deregulated microRNA (miRNA) function to carcinogenesis, relatively few miRNA-cancer gene interactions have been validated, making it difficult to appreciate the true complexity of miRNA-cancer gene regulatory networks.

Results

In this effort, we identify miRNA interactomes of 17 well-established cancer genes, involved in various cancer types, through a miRNome-wide 3' UTR reporter screening. Using a novel and performant strategy for high-throughput screening data analysis, we identify 390 interactions, quadrupling the size of the known miRNA interactome for the cancer genes under investigation. Clear enrichments of established and predicted interactions underscore the validity of the interactome data set. Interactomes app

SUBMITTER: Van Peer G 

PROVIDER: S-EPMC5844555 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature