Adrenocorticotropic hormone and 1,25-dihydroxyvitamin D₃ enhance human osteogenesis in vitro by synergistically accelerating the expression of bone-specific genes.

ABSTRACT: To improve definition of the physical and hormonal support of bone formation, we studied differentiation of human osteoblasts in vitro at varying combinations of ACTH, 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D), and extracellular calcium, with and without added cortisol. Bone mineralization, alkaline phosphatase activity, and osteoblast-specific markers RunX2, osterix, and collagen I increased with 10 pM ACTH, 10 nM 1,25(OH)₂D, or at 2 mM calcium with important synergistic activity of combinations of any of these stimuli. Signals induced by ACTH at 10-30 min included cAMP, TGF-β, and Erk1/2 phosphorylation. Affymetrix gene expression analysis showed that 2 h treatment of ACTH or 1,25(OH)₂D increased the expression of bone regulating and structural mR

SUBMITTER: Tourkova IL 

PROVIDER: S-EPMC5844701 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature