Rapid Intraoperative Diagnosis of Pediatric Brain Tumors Using Stimulated Raman Histology.

ABSTRACT: Accurate histopathologic diagnosis is essential for providing optimal surgical management of pediatric brain tumors. Current methods for intraoperative histology are time- and labor-intensive and often introduce artifact that limit interpretation. Stimulated Raman histology (SRH) is a novel label-free imaging technique that provides intraoperative histologic images of fresh, unprocessed surgical specimens. Here we evaluate the capacity of SRH for use in the intraoperative diagnosis of pediatric type brain tumors. SRH revealed key diagnostic features in fresh tissue specimens collected from 33 prospectively enrolled pediatric type brain tumor patients, preserving tumor cytology and histoarchitecture in all specimens. We simulated an intraoperative consultation for 25 patients with specimens

SUBMITTER: Hollon TC 

PROVIDER: S-EPMC5844703 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature