Hospital time prior to death and pancreas histopathology: implications for future studies.

ABSTRACT:

Aims/hypothesis

Diabetes research studies routinely rely upon the use of tissue samples from human organ donors. It remains unclear whether the length of hospital stay prior to organ donation affects the presence of cells infiltrating the pancreas or the frequency of replicating beta cells.

Methods

To address this, 39 organ donors without diabetes were matched for age, sex, BMI and ethnicity in groups of three. Within each group, donors varied by length of hospital stay immediately prior to organ donation (<3 days, 3 to <6 days, or ≥6 days). Serial sections from tissue blocks in the pancreas head, body and tail regions were immunohistochemically double stained for insulin and CD45, CD68, or Ki67. Slides were electronically scanned and quantitatively analysed for cell positiv

SUBMITTER: Kusmartseva I 

PROVIDER: S-EPMC5844815 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature