Small-RNA sequencing identifies dynamic microRNA deregulation during skeletal muscle lineage progression.

ABSTRACT: Skeletal muscle satellite cells are quiescent adult resident stem cells that activate, proliferate and differentiate to generate myofibres following injury. They harbour a robust proliferation potential and self-renewing capacity enabling lifelong muscle regeneration. Although several classes of microRNAs were shown to regulate adult myogenesis, systematic examination of stage-specific microRNAs during lineage progression from the quiescent state is lacking. Here we provide a genome-wide assessment of the expression of small RNAs during the quiescence/activation transition and differentiation by RNA-sequencing. We show that the majority of small RNAs present in quiescent, activated and differentiated muscle cells belong to the microRNA class. Furthermore, by comparing expression in distinc

SUBMITTER: Castel D 

PROVIDER: S-EPMC5844870 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature