Pathologic and biochemical characterization of PrP^Sc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent.

ABSTRACT:

Background

The Rocky Mountain elk (Cervus elaphus nelsoni) prion protein gene (PRNP) is polymorphic at codon 132, with leucine (L132) and methionine (M132) allelic variants present in the population. In elk experimentally inoculated with the chronic wasting disease (CWD) agent, different incubation periods are associated with PRNP genotype: LL132 elk survive the longest, LM132 elk are intermediate, and MM132 elk the shortest. The purpose of this study was to investigate potential mechanisms underlying variations in incubation period in elk of different prion protein genotypes. Elk calves of three PRNP genotypes (n = 2 MM132, n = 2 LM132, n = 4 LL132) were orally inoculated with brain homogenate from elk clinically affected with CWD.

Results

Elk with longer incubation periods

SUBMITTER: Moore SJ 

PROVIDER: S-EPMC5845354 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature