¹³C metabolic flux analysis of microbial and mammalian systems is enhanced with GC-MS measurements of glycogen and RNA labeling.

ABSTRACT: ¹³C metabolic flux analysis (¹³C-MFA) is a widely used tool for quantitative analysis of microbial and mammalian metabolism. Until now, ¹³C-MFA was based mainly on measurements of isotopic labeling of amino acids derived from hydrolyzed biomass proteins and isotopic labeling of extracted intracellular metabolites. Here, we demonstrate that isotopic labeling of glycogen and RNA, measured with gas chromatography-mass spectrometry (GC-MS), provides valuable additional information for ¹³C-MFA. Specifically, we demonstrate that isotopic labeling of glucose moiety of glycogen and ribose moiety of RNA greatly enhances resolution of metabolic fluxes in the upper part of metabolism; importantly, these measurements allow precise quantification of net and e

SUBMITTER: Long CP 

PROVIDER: S-EPMC5845444 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature