Metabolism of the fast-growing bacterium Vibrio natriegens elucidated by ¹³C metabolic flux analysis.

ABSTRACT: Vibrio natriegens is a fast-growing, non-pathogenic bacterium that is being considered as the next-generation workhorse for the biotechnology industry. However, little is known about the metabolism of this organism which is limiting our ability to apply rational metabolic engineering strategies. To address this critical gap in current knowledge, here we have performed a comprehensive analysis of V. natriegens metabolism. We constructed a detailed model of V. natriegens core metabolism, measured the biomass composition, and performed high-resolution ¹³C metabolic flux analysis (¹³C-MFA) to estimate intracellular fluxes using parallel labeling experiments with the optimal tracers [1,2-¹³C]glucose and [1,6-¹³C]glucose. During exponential growth in g

SUBMITTER: Long CP 

PROVIDER: S-EPMC5845447 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature