Genome-Wide Identification and Characterization of Long Non-Coding RNA in Wheat Roots in Response to Ca²⁺ Channel Blocker.

ABSTRACT: It remains unclear whether plant lncRNAs are responsive to Ca²⁺-channel blocking. When using the Ca²⁺-channel blocker, LaCl₃, to treat germinated wheat seeds for 24 h, we found that both root length and mitosis were inhibited in the LaCl₃-treated groups. The effect of the Ca²⁺-channel blocker was verified in three ways: a [Ca²⁺]_cyt decrease detected using Fluo-3/AM staining, a decrease in the Ca content measured using inductively coupled plasma mass spectrometry, and an inhibition of Ca²⁺ influx detected using Non-invasive Micro-test Technology. Genome-wide high throughput RNA-seq and bioinformatical methods were used to identify lncRNAs, and found 177 differentially expressed lncRNAs that might be in r

SUBMITTER: Ma K 

PROVIDER: S-EPMC5845709 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature