Functional analysis of the EsaB component of the <i>Staphylococcus aureus</i> Type VII secretion system.

ABSTRACT: Type VII secretion systems (T7SS) are found in many bacteria and secrete proteins involved in virulence and bacterial competition. In Staphylococcus aureus the small ubiquitin-like EsaB protein has been previously implicated as having a regulatory role in the production of the EsxC substrate. Here we show that in the S. aureus RN6390 strain, EsaB does not genetically regulate production of any T7 substrates or components, but is indispensable for secretion activity. Consistent with EsaB being an essential component of the T7SS, loss of either EsaB or EssC are associated with upregulation of a common set of iron acquisition genes. However, a further subset of genes were dysregulated only in the absence of EsaB. Quantitative western blotting indicates that EsaB is present at ve

SUBMITTER: Casabona MG 

PROVIDER: S-EPMC5845737 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature