Human ex vivo prostate tissue model system identifies ING3 as an oncoprotein.

ABSTRACT:

Background

Although the founding members of the INhibitor of Growth (ING) family of histone mark readers, ING1 and ING2, were defined as tumour suppressors in animal models, the role of other ING proteins in cellular proliferation and cancer progression is unclear.

Methods

We transduced ex vivo benign prostate hyperplasia tissues with inducible lentiviral particles to express ING proteins. Proliferation was assessed by H3S10^phos immunohistochemistry (IHC). The expression of ING3 was assessed by IHC on a human prostate cancer tissue microarray (TMA). Gene expression was measured by DNA microarray and validated by real-time qPCR.

Results

We found that ING3 stimulates cellular proliferation in ex vivo tissues, suggesting that ING3 could be oncogenic. Indeed, I

SUBMITTER: McClurg UL 

PROVIDER: S-EPMC5846061 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature