The glycosylation pathway is required for the secretion of Slit and for the maintenance of the Slit receptor Robo on axons.

ABSTRACT: Slit proteins act as repulsive axon guidance cues by activating receptors of the Roundabout (Robo) family. During early neurogenesis in Drosophila melanogaster, Slit prevents the growth cones of longitudinal tract neurons from inappropriately crossing the midline, thus restricting these cells to trajectories parallel to the midline. Slit is expressed in midline glial cells, and Robo is present in longitudinal axon tracts and growth cones. We showed that the enzyme Mummy (Mmy) controlled Slit-Robo signaling through mechanisms that affected both the ligand and the receptor. Mmy was required for the glycosylation of Slit, which was essential for Slit secretion. Mmy was also required for maintaining the abundance and spatial distribution of Robo through an indirect mechanism that was independe

SUBMITTER: Manavalan MA 

PROVIDER: S-EPMC5846327 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature