Inhibition of cell proliferation and migration through nucleobase-modified polyamidoamine-mediated p53 delivery.

ABSTRACT:

Introduction

The nucleobase 2-amino-6-chloropurine-modified polyamidoamine (AP-PAMAM) was used as a carrier for p53 gene delivery to achieve the antitumor effects.

Methods and materials

The condensation of p53 plasmid was studied through gel retardation assay, and the transfection efficiency was evaluated through the transfection assay of pEGFP-N3 and pGL-3 plasmids. Using human cervical carcinoma cell line HeLa as a model, the inhibition of cell proliferation and migration was studied through flow cytometry, wound healing and Transwell migration assays, respectively. The p53 expression level was detected through quantitative polymerase chain reaction and Western blotting analyses.

Results

The carrier could condense p53 plasmid into stable nanopar

SUBMITTER: Han H 

PROVIDER: S-EPMC5846749 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature