Protease signaling through protease activated receptor 1 mediate nerve activation by mucosal supernatants from irritable bowel syndrome but not from ulcerative colitis patients.

ABSTRACT:

Background & aims

The causes of gastrointestinal complaints in irritable bowel syndrome (IBS) remain poorly understood. Altered nerve function has emerged as an important pathogenic factor as IBS mucosal biopsy supernatants consistently activate enteric and sensory neurons. We investigated the neurally active molecular components of such supernatants from patients with IBS and quiescent ulcerative colitis (UC).

Method

Effects of supernatants from 7 healthy controls (HC), 20 IBS and 12 UC patients on human and guinea pig submucous neurons were studied with neuroimaging techniques. We identify differentially expressed proteins with proteome analysis.

Results

Nerve activation by IBS supernatants was prevented by the protease activated receptor 1 (PAR1) antagonist SCHE797

SUBMITTER: Buhner S 

PROVIDER: S-EPMC5846775 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature