Transformation of Accessible Chromatin and 3D Nucleome Underlies Lineage Commitment of Early T Cells.

ABSTRACT: How chromatin reorganization coordinates differentiation and lineage commitment from hematopoietic stem and progenitor cells (HSPCs) to mature immune cells has not been well understood. Here, we carried out an integrative analysis of chromatin accessibility, topologically associating domains, AB compartments, and gene expression from HSPCs to CD4⁺CD8⁺ T cells. We found that abrupt genome-wide changes at all three levels of chromatin organization occur during the transition from double-negative stage 2 (DN2) to DN3, accompanying the T lineage commitment. The transcription factor BCL11B, a critical regulator of T cell commitment, is associated with increased chromatin interaction, and Bcl11b deletion compromised chromatin interaction at its target genes. We propose that

SUBMITTER: Hu G 

PROVIDER: S-EPMC5847274 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature