Defining drug and target protein distributions after stent-based drug release: Durable versus deployable coatings.

ABSTRACT: BACKGROUND:Innovations in drug eluting stent designs make it increasingly important to develop models for differentiating performance through spatial definition of drug, receptor binding and cell state. METHODS:Two designs of sirolimus analog eluting stents were implanted into porcine coronary arteries for 28, 60 or 90 days (n = 9/time point), durable coating (Xience) and deployable absorbable coating (MiStent). Explanted arteries were evaluated for drug content (n = 3/time point) by LC-MS/MS and for drug and target protein (mTOR) distributions by immunofluorescence (IF, n = 6/time point). A computational model was developed to predict drug release and arterial distribution maps. RESULTS:Both stents released the majority of drug load by 28 days, with different tissue retention efficiencies

SUBMITTER: Tzafriri AR 

PROVIDER: S-EPMC5847480 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature