Time-lapse observation of stepwise regression of Erk activity in zebrafish presomitic mesoderm.

ABSTRACT: During somite segmentation, clock genes oscillate within the posterior presomitic mesoderm (PSM). The temporal information ties up with the posteriorly moving FGF gradient, leading to the formation of a presumptive somite within the PSM. We previously investigated Erk activity downstream of FGF signaling by collecting stained zebrafish embryos, and discovered that the steep gradient of Erk activity was generated in the PSM, and the Erk activity border regularly shifted in a stepwise manner. However, since these interpretations come from static analyses, we needed to firmly confirm them by applying an analysis that has higher spatiotemporal resolutions. Here we developed a live imaging system for Erk activity in zebrafish embryos, using a Förster resonance energy transfer (FRET)-based Erk b

SUBMITTER: Sari DWK 

PROVIDER: S-EPMC5847612 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature