Genome-Wide Identification by Transposon Insertion Sequencing of Escherichia coli K1 Genes Essential for <i>In Vitro</i> Growth, Gastrointestinal Colonizing Capacity, and Survival in Serum.

ABSTRACT: Escherichia coli K1 strains are major causative agents of invasive disease of newborn infants. The age dependency of infection can be reproduced in neonatal rats. Colonization of the small intestine following oral administration of K1 bacteria leads rapidly to invasion of the blood circulation; bacteria that avoid capture by the mesenteric lymphatic system and evade antibacterial mechanisms in the blood may disseminate to cause organ-specific infections such as meningitis. Some E. coli K1 surface constituents, in particular the polysialic acid capsule, are known to contribute to invasive potential, but a comprehensive picture of the factors that determine the fully virulent phenotype has not emerged so far. We constructed a library and constituent sublibraries of ∼775,000 Tn<

SUBMITTER: McCarthy AJ 

PROVIDER: S-EPMC5847654 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature