Peptide Transporter CstA Imports Pyruvate in Escherichia coli K-12.

ABSTRACT: Pyruvate is an important intermediate of central carbon metabolism and connects a variety of metabolic pathways in Escherichia coli Although the intracellular pyruvate concentration is dynamically altered and tightly balanced during cell growth, the pyruvate transport system remains unclear. Here, we identified a pyruvate transporter in E. coli using high-throughput transposon sequencing. The transposon mutant library (a total of 5 × 10⁵ mutants) was serially grown with a toxic pyruvate analog (3-fluoropyruvate [3FP]) to enrich for transposon mutants lacking pyruvate transport function. A total of 52 candidates were selected on the basis of a stringent enrichment level of transposon insertion frequency in response to 3FP treatment. Subsequently, their pyruvate tran

SUBMITTER: Hwang S 

PROVIDER: S-EPMC5847655 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature