Assessment of reactive oxygen species generated by electronic cigarettes using acellular and cellular approaches.

ABSTRACT: Electronic cigarettes (e-cigs) have fast increased in popularity but the physico-chemical properties and toxicity of the generated emission remain unclear. Reactive oxygen species (ROS) are likely present in e-cig emission and can play an important role in e-cig toxicity. However, e-cig ROS generation is poorly documented. Here, we generated e-cig exposures using a recently developed versatile exposure platform and performed systematic ROS characterization on e-cig emissions using complementary acellular and cellular techniques: 1) a novel acellular Trolox-based mass spectrometry method for total ROS and hydrogen peroxide (H₂O₂) detection, 2) electron spin resonance (ESR) for hydroxyl radical detection in an acellular and cellular systems and 3) in vitro ROS detection

SUBMITTER: Zhao J 

PROVIDER: S-EPMC5848214 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature