Transcriptome analysis of pig intestinal cell monolayers infected with Cryptosporidium parvum asexual stages.

ABSTRACT:

Background

Human cryptosporidiosis is caused primarily by two species of apicomplexan protozoa, Cryptosporidium parvum and C. hominis. In cultured cell monolayers, the parasite undergoes two generations of asexual multiplication (merogony). However, the proportion of parasites completing the life-cycle is low and insufficient to sustain continuous propagation. Due to the intracellular location of meronts and later life-cycle stages, oocyst and sporozoites are the only forms of the parasite that can readily be isolated.

Results

Research on the replicating forms of Cryptosporidium parasites and their interaction with the host cell remains challenging. Based on an RNA-Seq analysis of monolayers of pig epithelial cells infected with C. parvum, here we report on the impact of mer

SUBMITTER: Mirhashemi ME 

PROVIDER: S-EPMC5848449 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature