Deregulated microRNA and mRNA expression profiles in the peripheral blood of patients with Marfan syndrome.

ABSTRACT: BACKGROUND:MicroRNAs (miRNAs) are small RNAs regulating gene expression post-transcriptionally. While acquired changes of miRNA and mRNA profiles in cancer have been extensively studied, little is known about expression changes of circulating miRNAs and messenger RNAs (mRNA) in monogenic constitutional anomalies affecting several organ systems, like Marfan syndrome (MFS). We performed integrated miRNA and mRNA expression profiling in blood samples of Marfan patients in order to investigate deregulated miRNA and mRNA networks in these patients which could serve as potential diagnostic and prognostic tools for MFS therapy. METHODS:MiRNA and mRNA expression profiles were determined in blood samples from MFS patients (n?=?7) and from healthy volunteer controls (n?=?7) by microarray analysis. Enrichment analyses of altered mRNA expression were identified using bioinformatic tools. RESULTS:A total of 28 miRNAs and 32 mRNAs were found to be significantly altered in MFS patients compared to controls (>?2.0-fold change, adjusted P?

SUBMITTER: Abu-Halima M 

PROVIDER: S-EPMC5848586 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature