Genetic diversity within dominant Enterocytozoon bieneusi genotypes in pre-weaned calves.

ABSTRACT: BACKGROUND:Cattle are commonly infected with the microsporidian parasite Enterocytozoon bieneusi. Sequence characterization of E. bieneusi in these animals at the ribosomal internal transcribed spacer (ITS) locus had identified I, J and BEB4 as the dominant genotypes. However, current studies on E. bieneusi in dairy cattle are mostly on infection rates and genotype distribution. This study aims to examine the intragenotypic diversity within dominant E. bieneusi genotypes in pre-weaned dairy calves in Shanghai, China. METHODS:Enterocytozoon bieneusi genotypes and subtypes were identified by PCR sequence analysis of ITS and multilocus sequence typing (MLST), based on material from farms. Chi-square test was used to examine differences in E. bieneusi infection rates between farms or age group

SUBMITTER: Tang C 

PROVIDER: S-EPMC5848593 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature