Adsorption of bovine serum albumin on silicon dioxide nanoparticles: Impact of pH on nanoparticle-protein interactions.

ABSTRACT: Bovine serum albumin (BSA) adsorbed on amorphous silicon dioxide (SiO₂) nanoparticles was studied as a function of pH across the range of 2 to 8. Aggregation, surface charge, surface coverage, and protein structure were investigated over this entire pH range. SiO₂ nanoparticle aggregation is found to depend upon pH and differs in the presence of adsorbed BSA. For SiO₂ nanoparticles truncated with hydroxyl groups, the largest aggregates were observed at pH 3, close to the isoelectric point of SiO₂ nanoparticles, whereas for SiO₂ nanoparticles with adsorbed BSA, the aggregate size was the greatest at pH 3.7, close to the isoelectric point of the BSA-SiO₂ complex. Surface coverage of BSA was also the greatest at the isoelectric

SUBMITTER: Givens BE 

PROVIDER: S-EPMC5848789 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature