Proteome evaluation of human cystic echinococcosis sera using two dimensional gel electrophoresis.

ABSTRACT:

Aim

Detection of protein expression changes in human cystic echinococcosis sera by 2D gel electrophoresis.

Background

Diagnosis and successful treatment of cystic echinococcosis (CE) is a major challenge, up to now. Identification of related expressed proteins using proteomics tools and bioinformatics analysis of patients' sera have not been investigated, so far.

Methods

Sera from eight confirmed CE patients and three healthy controls were collected, tested by 2-DE for total protein separation of serum and analyzed using proteomics and bioinformatics methods. The gels were stained by Coomassie blue followed by scan imaging of the gels. The protein spots in each gel were analyzed using progenesis same spots software. Proteins names were obtained from TagIdent server.

SUBMITTER: Sadjjadi FS 

PROVIDER: S-EPMC5849122 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature