X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds.

ABSTRACT: Crystallization of membrane proteins is a critical step for uncovering atomic resolution 3-D structures and elucidating structure-function relationships. Microseeding, the process of transferring sub-microscopic crystal nuclei from initial screens into new crystallization experiments, is an effective, yet underutilized approach to grow crystals suitable for X-ray crystallography. Here, we report simplified methods for crystallization of membrane proteins that utilize microseeding in X-ray transparent microfluidic chips. First, a microfluidic method for introduction of microseed dilutions into metastable crystallization experiments is demonstrated for photoactive yellow protein and cytochrome bo3 oxidase. As microseed concentration decreased, the number of crystals decreased while the avera

SUBMITTER: Schieferstein JM 

PROVIDER: S-EPMC5849577 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature