Defining the Transcriptional Landscape during Cytomegalovirus Latency with Single-Cell RNA Sequencing.

ABSTRACT: Primary infection with human cytomegalovirus (HCMV) results in a lifelong infection due to its ability to establish latent infection, with one characterized viral reservoir being hematopoietic cells. Although reactivation from latency causes serious disease in immunocompromised individuals, our molecular understanding of latency is limited. Here, we delineate viral gene expression during natural HCMV persistent infection by analyzing the massive transcriptome RNA sequencing (RNA-seq) atlas generated by the Genotype-Tissue Expression (GTEx) project. This systematic analysis reveals that HCMV persistence in vivo is prevalent in diverse tissues. Notably, we find only viral transcripts that resemble gene expression during various stages of lytic infection with no evidence of any highly

SUBMITTER: Shnayder M 

PROVIDER: S-EPMC5850328 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature