Dose-Dependent Effects on Replication and Persistence of Salmonella enterica serovar Typhimurium in House Flies (Diptera: Muscidae).

ABSTRACT: Adult house flies (Musca domestica L.) ingest variable numbers of bacteria when they encounter microbe-rich substrates. Bacterial abundance may affect replication within the fly gut, which subsequently impacts vector potential. This study investigated the dose-dependent replication of GFP-expressing Salmonella enterica serovar Typhimurium (ex Kauffmann and Edwards1952) Le Minor and Popoff 1987, (Enterobacteriales: Enterobacteriaceae) (GFP S. Typhimurium) within the fly alimentary canal. Adult house flies were fed two doses (colony forming units, CFU) of GFP S. Typhimurium (high, ~105 CFU and low, ~104 CFU). Bacteria were examined at 2-, 4-, 6-, 12-, and 24-h postingestion (PI) in situ in the gut via epifluorescence microscopy and enumerated by culture on selective media. In both treatment

SUBMITTER: Chifanzwa R 

PROVIDER: S-EPMC5850332 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature