High Whole-Genome Sequence Diversity of Human Papillomavirus Type 18 Isolates.

ABSTRACT:

Background

The most commonly found human papillomavirus (HPV) types in cervical cancer are HPV16 and HPV18. Genome variants of these types have been associated with differential carcinogenic potential. To date, only a handful of studies have described HPV18 whole genome sequencing results. Here we describe HPV18 variant diversity and conservation of persistent infections in a longitudinal retrospective cohort study.

Methods

Cervical self-samples were obtained annually over four years and genotyped on the SPF10-DEIA-LiPA₂₅ platform. Clearing and persistent HPV18 positive infections were selected, amplified in two overlapping fragments, and sequenced using 32 sequence primers.

Results

Complete viral genomes were obtained from 25 participants with persistent a

SUBMITTER: van der Weele P 

PROVIDER: S-EPMC5850375 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature