Transcriptional profiles of JIA patient blood with subsequent poor response to methotrexate.

ABSTRACT:

Objective

The mechanisms that determine the efficacy or inefficacy of MTX in JIA are ill-defined. The objective of this study was to identify a gene expression transcriptional signature associated with poor response to MTX in patients with JIA.

Methods

RNA sequencing was used to measure gene expression in peripheral blood mononuclear cells collected from 47 patients with JIA prior to MTX treatment and 14 age-matched controls. Differentially expressed baseline genes between responders and non-responders were evaluated. Biological differences between all JIA patients and controls were explored by constructing a signature of differentially expressed genes. Unsupervised clustering and pathway analysis was performed.

Results

A signature of 99 differentially expressed genes

SUBMITTER: Moncrieffe H 

PROVIDER: S-EPMC5850489 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature