LNA Thymidine Monomer Enables Differentiation of the Four Single-Nucleotide Variants by Melting Temperature.

ABSTRACT: High-resolution melting (HRM) analysis of DNA is a closed-tube single-nucleotide polymorphism (SNP) detection method that has shown many advantages in point-of-care diagnostics and personalized medicine. While recently developed melting probes have demonstrated significantly improved discrimination of mismatched (mutant) alleles from matched (wild-type) alleles, no effort has been made to design a simple melting probe that can reliably distinguish all four SNP alleles in a single experiment. Such a new probe could facilitate the discovery of rare genetic mutations at lower cost. Here we demonstrate that a melting probe embedded with a single locked thymidine monomer (t_L) can reliably differentiate the four SNP alleles by four distinct melting temperatures (termed the "4T_m</

SUBMITTER: Obliosca JM 

PROVIDER: S-EPMC5850941 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature