CoIN: co-inducible nitrate expression system for secondary metabolites in Aspergillus nidulans.

ABSTRACT:

Background

Sequencing of fungal species has demonstrated the existence of thousands of putative secondary metabolite gene clusters, the majority of them harboring a unique set of genes thought to participate in production of distinct small molecules. Despite the ready identification of key enzymes and potential cluster genes by bioinformatics techniques in sequenced genomes, the expression and identification of fungal secondary metabolites in the native host is often hampered as the genes might not be expressed under laboratory conditions and the species might not be amenable to genetic manipulation. To overcome these restrictions, we developed an inducible expression system in the genetic model Aspergillus nidulans.

Results

We genetically engineered a strain of A.

SUBMITTER: Wiemann P 

PROVIDER: S-EPMC5851313 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature