In silico modeling of epigenetic-induced changes in photoreceptor cis-regulatory elements.

ABSTRACT: Purpose:DNA methylation is a well-characterized epigenetic repressor of mRNA transcription in many plant and vertebrate systems. However, the mechanism of this repression is not fully understood. The process of transcription is controlled by proteins that regulate recruitment and activity of RNA polymerase by binding to specific cis-regulatory sequences. Cone-rod homeobox (CRX) is a well-characterized mammalian transcription factor that controls photoreceptor cell-specific gene expression. Although much is known about the functions and DNA binding specificity of CRX, little is known about how DNA methylation modulates CRX binding affinity to genomic cis-regulatory elements. Methods:We used bisulfite pyrosequencing of human ocular tissues to measure DNA methylation levels of the regulatory

SUBMITTER: Hossain RA 

PROVIDER: S-EPMC5851326 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature