Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX.

ABSTRACT: Microbial secondary metabolites represent a rich source of valuable compounds with a variety of applications in medicine or agriculture. Effective exploitation of this wealth of chemicals requires the functional expression of the respective biosynthetic genes in amenable heterologous hosts. We have previously established the TREX system which facilitates the transfer, integration and expression of biosynthetic gene clusters in various bacterial hosts. Here, we describe the yTREX system, a new tool adapted for one-step yeast recombinational cloning of gene clusters. We show that with yTREX, Pseudomonas putida secondary metabolite production strains can rapidly be constructed by random targeting of chromosomal promoters by Tn5 transposition. Feasibility of this approach was corroborated by p

SUBMITTER: Domrose A 

PROVIDER: S-EPMC5851919 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature