Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression.

ABSTRACT: Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway engineering. Along the way, combinatorial gene expression control through modification of regulatory element offered tremendous opportunity for fine-tuning gene expression and generating digital-like genetic circuits. In this report, we present an efficient evolutionary approach to build a range of regulatory control elements. The reported method allows for rapid construction of promoter, 5'UTR, terminator and trans-activating RNA libraries. Synthetic overlapping oligos with high portion of degenerate nucleotides flanking the regulatory element could be efficiently assembled to a vector expressing fluorescenc

SUBMITTER: Jin E 

PROVIDER: S-EPMC5851936 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature