Wet milling of large quantities of human excision adipose tissue for the isolation of stromal vascular fraction cells.

ABSTRACT: The isolation of stromal vascular fraction (SVF) cells from excised human adipose tissue, for clinical or research purposes, implies the tedious and time consuming process of manual mincing prior to enzymatic digestion. Since no efficient alternative technique to this current standard procedure has been proposed so far, the aim of this study was to test a milling procedure, using two simple, inexpensive and commercially available manual meat grinders, to process large amounts of adipose tissue. The procedure was assessed on adipose tissue resections from seven human donors and compared to manual mincing with scalpels. The processed adipose tissues were digested and the resulting SVF cells compared in terms of number, clonogenicity and differentiation capacity. After 10 min of processing, e

SUBMITTER: Menzi N 

PROVIDER: S-EPMC5851973 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature