Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates.

ABSTRACT: Bio-imaging is a key technique in tracking and monitoring important biological processes and fundamental biomolecular interactions, however the interference of background autofluorescence with targeted fluorophores is problematic for many bio-imaging applications. This study reports on two novel methods for reducing interference with cellular autofluorescence for bio-imaging. The first method uses fluorescent nanodiamonds (FNDs), containing nitrogen vacancy centers. FNDs emit at near-infrared wavelengths typically higher than most cellular autofluorescence; and when appropriately functionalized, can be used for background-free imaging of targeted biomolecules. The second method uses europium-chelating tags with long fluorescence lifetimes. These europium-chelating tags enhance background-f

SUBMITTER: Cordina NM 

PROVIDER: S-EPMC5851999 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature