Microvillar and ciliary defects in zebrafish lacking an actin-binding bioactive peptide amidating enzyme.

ABSTRACT: The assembly of membranous extensions such as microvilli and cilia in polarized cells is a tightly regulated, yet poorly understood, process. Peptidylglycine α-amidating monooxygenase (PAM), a membrane enzyme essential for the synthesis of amidated bioactive peptides, was recently identified in motile and non-motile (primary) cilia and has an essential role in ciliogenesis in Chlamydomonas, Schmidtea and mouse. In mammalian cells, changes in PAM levels alter secretion and organization of the actin cytoskeleton. Here we show that lack of Pam in zebrafish recapitulates the lethal edematous phenotype observed in Pam ^-/- mice and reveals additional defects. The pam ^-/- zebrafish embryos display an initial striking loss of microvilli and subsequently impaired ciliogenesis

SUBMITTER: Kumar D 

PROVIDER: S-EPMC5852006 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature